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Questions related from Lael Werner
Anyone familiar with a method to quantify an image generated by 2 photon microscope? It is intestinal human biopsies.
27 February 2018 6,126 4 View
Hello. I am using Miltenyi's Magnetic Treg isolation (CD4+CD25+CD127 dim). Does any one have experience if I can use the LS column for the 1st isolation, instead of the LD depletion columns? What...
28 February 2017 243 1 View
Hello. Need help with (eBioscience) FoxP3 kit. I heard the external antibodies (CD4 and CD25) can be added, AFTER the fix/perm, after overnight incubation at 4C, CONCURRENTLY with the Foxp3...
04 January 2017 8,465 5 View
How much IL-10 can be expected to secreted from 1million (freshly isolated and magnetically seperated, human) CD4+ cells, stimulated for 24 and/or 48 hours 1ug/ml plated CD3+ 2ug/ml soluble...
21 December 2016 1,930 2 View
I am looking to isolate whole, viable cells from human intestinal resections, using the MACS homogenizer. Does any one have experience with it for whole-cell, single suspension of cells? What is...
21 November 2016 8,545 0 View
I have been activating T cells with anti-CD3 (either soluble or plate bound) for many years. Currently, I am establishing a protocol whereby at the time of coating (or subsequently) I plate bind...
20 October 2016 5,053 1 View