Lael Werner

6 Questions 12 Answers 0 Followers

Questions related from Lael Werner

Method for quantification of 2 photon Microscopy?

Anyone familiar with a method to quantify an image generated by 2 photon microscope? It is intestinal human biopsies.

27 February 2018 6,126 4 View

Treg Isolation kit?

Hello. I am using Miltenyi's Magnetic Treg isolation (CD4+CD25+CD127 dim). Does any one have experience if I can use the LS column for the 1st isolation, instead of the LD depletion columns? What...

28 February 2017 244 1 View

Experience with Foxp3 intracellular staining?

Hello. Need help with (eBioscience) FoxP3 kit. I heard the external antibodies (CD4 and CD25) can be added, AFTER the fix/perm, after overnight incubation at 4C, CONCURRENTLY with the Foxp3...

04 January 2017 8,467 5 View

How much IL-10 secreted from 1million (isolated) CD4+ cells?

How much IL-10 can be expected to secreted from 1million (freshly isolated and magnetically seperated, human) CD4+ cells, stimulated for 24 and/or 48 hours 1ug/ml plated CD3+ 2ug/ml soluble...

21 December 2016 1,933 2 View

Any experience with MACS homogenizer?

I am looking to isolate whole, viable cells from human intestinal resections, using the MACS homogenizer. Does any one have experience with it for whole-cell, single suspension of cells? What is...

21 November 2016 8,545 0 View

Anti-CD3 for stimulation is displaced?

I have been activating T cells with anti-CD3 (either soluble or plate bound) for many years. Currently, I am establishing a protocol whereby at the time of coating (or subsequently) I plate bind...

20 October 2016 5,055 1 View