Protocol for fixing and mounting fungi from agar culture?

I'm looking for a good protocol for taking fungi (mostly conidial ascomycetes) growing on an agar plate and fixing them and ultimately staining and mounting them. Would one simply remove a plug of...

03 June 2019 8,227 5 View

Gelatin as a gelling agent for fungal growth media?

I'm planning an experiment involving imaging cultures of mycoparasitic Trichodermium interacting with various species of host fungi and am thinking of various methods of imaging the interaction...

26 May 2019 2,466 3 View

How to alter dimer-monomer equilibrium in proteins?

I have a protein forming a very stable dimer in solution, but still running as monomer in SDS page. I have tryed to disrupt the dimer with Beta-ME and high salt, but it is still running as dimmer...

21 January 2018 2,921 3 View

Can a short burst of electricity cause irreversible membrane damage to Ecoli strains?

No growth observed on plates and I can't explain why as the sample was only subjected to current for a second.

10 November 2017 4,016 1 View

Experience with Foxp3 intracellular staining?

Hello. Need help with (eBioscience) FoxP3 kit. I heard the external antibodies (CD4 and CD25) can be added, AFTER the fix/perm, after overnight incubation at 4C, CONCURRENTLY with the Foxp3...

03 January 2017 8,353 5 View

How much IL-10 secreted from 1million (isolated) CD4+ cells?

How much IL-10 can be expected to secreted from 1million (freshly isolated and magnetically seperated, human) CD4+ cells, stimulated for 24 and/or 48 hours 1ug/ml plated CD3+ 2ug/ml soluble...

20 December 2016 1,837 2 View

Anti-CD3 for stimulation is displaced?

I have been activating T cells with anti-CD3 (either soluble or plate bound) for many years. Currently, I am establishing a protocol whereby at the time of coating (or subsequently) I plate bind...

19 October 2016 4,989 1 View

How can I transform a data series with negative, zero, and positive data points?

The data series is from changes in bee hive weights per day, so all data points are important. They are not normally distributed (many zeros) hence the need to transform the series. I wanted to...

01 June 2016 7,183 7 View

Which CD133 epitope for identifying cancer stem cells; CD133/1 or Cd133/2?

05 May 2016 8,503 2 View

What are the best resources to aid in determining what questions to ask in a survey?

Survey resources focus on what surveys are and how they are constructed but does not provide guidance on how or what to translate from literature review or other research inputs into a survey. Are...

10 January 2016 7,384 10 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is the logarithmic transformation a necessary step in time series analysis ?

Hello, I'm checking if the log-tranformation of time series data is a necessary step; We all know the aim of such transformation, but in every study (in time series analysis) we found the authors...

15 February 2021 5,655 14 View