My database is SLAG 4. I know this database has some limitations, but that's what I have.
I'm challenging bacteria with different compounds, then plating the suspension on LB agar to assess CFU and understand the bactericidal properties of my compounds. However, I'm encountering...
10 April 2024 7,459 7 View
I am culturing CACO2 cells for experiments. I split them when they reach 80% confluence. what happens if they reach 100% confluence, and I keep splitting them? Confluence of CACO2 epithelial cells...
02 January 2024 9,198 1 View
I think that mentoring programmes support in meeting publishers' expectations.
05 October 2023 2,584 1 View
Does someone have experience with expression of Tight junction molecules in FACS? I am looking for ZO-1 and occludin expression on CACO2 epithelial cells. Any suggestions or recommendations?
21 August 2023 5,312 0 View
Hello! I am currently conducting a qualitative study about leadership styles where I need to determine the leadership style used by my participants and I need help in looking for an already...
22 June 2023 7,753 3 View
Are there methods for Quantification of C. Albicans in serum or stool, without culturing them? Some fluorimetric or ELISA based assay? If it can differentiate between Yeast and Hypahe, even better
11 June 2023 8,792 2 View
I was foolish enough to participate in a discussion about eponyms that for some reason I do not quite understand attracts a lot of activity in the community. I made a single short comment and now...
01 April 2023 8,093 3 View
I need to measure TEER in CACO2 cell, before and after 6 hours with some stimulation. How do I know if the cells are ready and confluent? In some articles, baseline TEER is 1000 OHM or higher. In...
28 March 2023 8,894 0 View
Dear community, does anyone has an idea, if or how to transform *.IMA data (spectroscopy!) to *.rda data, despite exportation to the "Spectroscopy" menu and save as *.rda? (nearly 240 data sets...
14 March 2023 9,016 0 View
I am looking for a semi-qualitative method to determine microbial oxygen usage in human stool samples. In Wikipedia, Thioglycolate broth is used for the differentiation of obligate aerobes,...
21 January 2023 988 1 View
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31 July 2024 1,671 3 View
Dear users, I would like to simulate a zeolite structure in gromacs. I got the .cif file from IZA expanded in one direction in Materials Studio and exported a .pdb file. However, when I want to...
24 July 2024 367 4 View
I want to simulate WRF for a 12*12 km domain size. I want to run LSM and BSM models with a 5 minutes time interval. My laptop specification is 16GB RAM and Intel i5-1235U 1.3 GHz processor. Can I...
13 July 2024 2,037 0 View
The diagram suggests that there is a contradiction within the second law of thermodynamics and Carnot's efficiency theory. According to the second law, Carnot efficiency is independent of the...
08 July 2024 7,478 4 View
Hi Dear All I would like to simulate a mode locked semiconductor laser. Does anyone know what is the best software to do it?
06 July 2024 1,648 4 View
I attempted to find a tutorial on YouTube but couldn't locate it. Could anyone guide me on how to view simulation results for Mobile Edge Computing using the OMNET++ simulator?
06 July 2024 6,229 0 View
I am looking at ways to investigate the biodegradation of 3D printed specimen as part of my research. To what extent can SimaPro be used to do this? The idea is to demonstrate the biodegradation...
02 July 2024 2,730 2 View
I used forcite module of Material Studio software to simulate the dissolution of zinc production waste in deep eutectic solvent. UFF force field has been used for the optimization of molecules,...
02 July 2024 7,494 1 View
I am trying to simulate a new solar cell which has almost 15 layers. But SCAPS has only 7 layers.
01 July 2024 9,135 2 View
Hello, Has anyone successfully modeled 2D argon plasma jet using the COMSOL? I would greatly appreciate any guidance on this matter.
30 June 2024 5,597 0 View