Confusing MTT result. Need Help anyone?

Currently im doing the cell viability test for 24h, 48h and 72h. On the 24h plate, the reading appeared confusing same as 48h plate. I have 7 extracts concentrations, 0.001%, 0.01%, 0.1%, 2%, 4%,...

04 May 2016 3,028 4 View

How do i evaluate from TEM and SEM micrograph for inflammation?

does we look for specific organelles? morphology changes of the cells?

09 October 2015 7,406 1 View

Anyone know where i can get a journal solely from water extracts?

i have been looking a water extract of plant without proceed to powder or any other method. A direct process of water extract and treat directly

05 June 2015 7,926 1 View

Anyone can suggest a good journal for electron microscopy?

a Q1 journal on microscopy.

04 May 2015 3,499 0 View

Does anyone have any references regarding aqueous extraction protocols?

04 May 2015 2,003 7 View

Does anyone have the data sheet for heparin (17513-54) from Nacalai Tesque?

31 December 2014 9,086 0 View

How to choose the right gene for vascular inflammation? any tools to look for these gene?

gene for tissue factor, IL 6 and 1-beta, TNF alpha

10 November 2014 1,883 2 View

Will there be any changes to the cell line if supplements given were not from same company?

10 November 2014 9,176 5 View

What are the most effective protocols to measure the tissue factor activities?

I need help from the experts to choose the reasonable protocols/methods on screening/evaluating/measure the tissue factors (TF) activities.

09 October 2014 6,704 2 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View