Amierul Sharudin

10 Questions 10 Answers 0 Followers

Questions related from Amierul Sharudin

Confusing MTT result. Need Help anyone?

Currently im doing the cell viability test for 24h, 48h and 72h. On the 24h plate, the reading appeared confusing same as 48h plate. I have 7 extracts concentrations, 0.001%, 0.01%, 0.1%, 2%, 4%,...

05 May 2016 3,057 4 View

How do i evaluate from TEM and SEM micrograph for inflammation?

does we look for specific organelles? morphology changes of the cells?

10 October 2015 7,436 1 View

Anyone know where i can get a journal solely from water extracts?

i have been looking a water extract of plant without proceed to powder or any other method. A direct process of water extract and treat directly

06 June 2015 7,956 1 View

Anyone can suggest a good journal for electron microscopy?

a Q1 journal on microscopy.

05 May 2015 3,523 0 View

Does anyone have any references regarding aqueous extraction protocols?

i need to understand of how the extracts will be carried out for treatment purposed e.g 200g of fruits soaks all night and later boiled in 5L of distilled water.

05 May 2015 2,031 7 View

Does anyone have the data sheet for heparin (17513-54) from Nacalai Tesque?

17513-54 from Nacalai Tesque

01 January 2015 9,113 0 View

How to choose the right gene for vascular inflammation? any tools to look for these gene?

gene for tissue factor, IL 6 and 1-beta, TNF alpha

11 November 2014 1,916 2 View

Will there be any changes to the cell line if supplements given were not from same company?

Has anyone experienced any problems by mixing supplements? For example, the cell line is from ATCC and the supplement is from Sigma. HUVECS (ATCC), ECGS (SIGMA), HEPARIN (NACALI TESQUE).

11 November 2014 9,205 5 View

What are the most effective protocols to measure the tissue factor activities?

I need help from the experts to choose the reasonable protocols/methods on screening/evaluating/measure the tissue factors (TF) activities.

10 October 2014 6,752 2 View

How many passage is ideal to use for cell culture and how to make the commercialized culture inflamed? what is the best media?

I would like to do a cellular level screening of the pro inflammatory activities. Any suggestion for reading, for a detail protocol and procedure?

02 February 2014 5,121 2 View