How to choose the right gene for vascular inflammation? any tools to look for these gene?

More Amierul Sharudin's questions See All

Confusing MTT result. Need Help anyone?

Currently im doing the cell viability test for 24h, 48h and 72h. On the 24h plate, the reading appeared confusing same as 48h plate. I have 7 extracts concentrations, 0.001%, 0.01%, 0.1%, 2%, 4%,...

04 May 2016 3,088 4 View

How do i evaluate from TEM and SEM micrograph for inflammation?

does we look for specific organelles? morphology changes of the cells?

09 October 2015 7,468 1 View

Anyone know where i can get a journal solely from water extracts?

i have been looking a water extract of plant without proceed to powder or any other method. A direct process of water extract and treat directly

05 June 2015 7,988 1 View

Anyone can suggest a good journal for electron microscopy?

a Q1 journal on microscopy.

04 May 2015 3,556 0 View

Does anyone have any references regarding aqueous extraction protocols?

i need to understand of how the extracts will be carried out for treatment purposed e.g 200g of fruits soaks all night and later boiled in 5L of distilled water.

04 May 2015 2,066 7 View

Does anyone have the data sheet for heparin (17513-54) from Nacalai Tesque?

17513-54 from Nacalai Tesque

31 December 2014 9,145 0 View

Will there be any changes to the cell line if supplements given were not from same company?

Has anyone experienced any problems by mixing supplements? For example, the cell line is from ATCC and the supplement is from Sigma. HUVECS (ATCC), ECGS (SIGMA), HEPARIN (NACALI TESQUE).

10 November 2014 9,240 5 View

What are the most effective protocols to measure the tissue factor activities?

I need help from the experts to choose the reasonable protocols/methods on screening/evaluating/measure the tissue factors (TF) activities.

09 October 2014 6,850 2 View

How many passage is ideal to use for cell culture and how to make the commercialized culture inflamed? what is the best media?

I would like to do a cellular level screening of the pro inflammatory activities. Any suggestion for reading, for a detail protocol and procedure?

01 February 2014 5,166 2 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What can i do to find the right topic for my thesis?

i am facing the difficulty to find the right and latest topic for my thesis. i searched and read hundreds of articles but i couldn't find the right one. what can i do I'm really confused.

02 August 2024 1,323 8 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Do we save MSMEs of the Nation ?

SMEs are backbone of the country's white economy, most of them are II generation entrepreneurs and migrated from family owned business policies, and having sufficient higher educations for...

01 August 2024 5,562 2 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

How to choose the journal?

Hello I want a suitable journal in the field of remote sensing and machine learning to be judged quickly. Thank you for your guidance Thanks

01 August 2024 1,799 4 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View