Confusing MTT result. Need Help anyone?

Currently im doing the cell viability test for 24h, 48h and 72h. On the 24h plate, the reading appeared confusing same as 48h plate. I have 7 extracts concentrations, 0.001%, 0.01%, 0.1%, 2%, 4%,...

04 May 2016 3,028 4 View

How do i evaluate from TEM and SEM micrograph for inflammation?

does we look for specific organelles? morphology changes of the cells?

09 October 2015 7,406 1 View

Anyone know where i can get a journal solely from water extracts?

i have been looking a water extract of plant without proceed to powder or any other method. A direct process of water extract and treat directly

05 June 2015 7,926 1 View

Anyone can suggest a good journal for electron microscopy?

a Q1 journal on microscopy.

04 May 2015 3,499 0 View

Does anyone have any references regarding aqueous extraction protocols?

04 May 2015 2,003 7 View

Does anyone have the data sheet for heparin (17513-54) from Nacalai Tesque?

31 December 2014 9,086 0 View

Will there be any changes to the cell line if supplements given were not from same company?

10 November 2014 9,176 5 View

What are the most effective protocols to measure the tissue factor activities?

I need help from the experts to choose the reasonable protocols/methods on screening/evaluating/measure the tissue factors (TF) activities.

09 October 2014 6,704 2 View

How many passage is ideal to use for cell culture and how to make the commercialized culture inflamed? what is the best media?

I would like to do a cellular level screening of the pro inflammatory activities. Any suggestion for reading, for a detail protocol and procedure?

01 February 2014 5,072 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View