Bobbi Fleiss

9 Questions 75 Answers 0 Followers

Questions related from Bobbi Fleiss

Reverse engineering a protein - kind of?

I want to bind a peptide to a hydrogel and test the release kinetics over time using an ELISA. BUT, there is no antibody/ELISA available for my peptide. So, I know that the molecular weight of my...

10 October 2018 6,409 4 View

Thalamic photothrombotic stroke - intersecting lasers?

Is it possible to use two intersecting lasers whose field strength is added at their point of intersection to activate a thrombotic dye? I would like to be able to target more than just the cortex...

11 November 2017 9,472 0 View

Paper using PSD95 antibody in a Dlg4 KO mouse ?

I have been asked by a reviewer to justify the specificity of a Dlg4 antibody - MA1-045 (6G6-1C9) from Thermo (previously millipore). Does anyone know in what paper someone has done the control...

10 October 2016 5,336 3 View

Does anyone know where I can get the list of genes which are known downstream targets for the transcription factor CEBPB?

Using Enricher I can see that my gene set contains 235/3478 genes known to be targets of CEBPB via the Transfac/Jasper tab. Does anyone know where i could get the list of all the CEBPB genes so...

02 February 2016 9,701 3 View

Does anyone know of research looking at the effects of repeated viral infection in first few weeks of life in a mouse to model childhood illnesses?

Does anyone know of any research looking at the effects of repeated viral infection in first few weeks of life in a mouse to model childhood illnesses (or similar)? Thanks!

11 November 2015 9,974 4 View

Stability of estradiol in PBS frozen at -20 - can anyone help?

Would I expect there to be a significant loss of bioactivity of E4 diluted in PBS and then frozen once before use? I would prefer to make a batch and then aliquot and store, as opposed to making...

09 September 2013 8,371 8 View

Anti-Iba1 antibody (ab5076) : Does anyone have any experience with it in mouse or monkey?

We are looking for a good alternative to the Rabbit-Anti-Iba1 from Wako as although it it great it limits the use of other rabbit antibodies for double labelling. Does anyone have any experience...

09 September 2013 7,793 14 View

Microglial Primary Culture- one brain at a time?

We have mixed genotype litters of pups that we would like to culture, but it is difficult to do the genotyping before culturing the microglia (either E19 or P0 pups) and we were wondering how...

07 July 2013 2,826 2 View

Is it beneficial to use ELISA to quantify p53 levels in cultured cell fractions?

ELISA and western blots are only as good as the antibodies used to make them - but despite a plethora of published westerns for measuring p53 there are very few reports using ELISA. I have...

11 November 2012 8,604 15 View