i need to understand of how the extracts will be carried out for treatment purposed
e.g 200g of fruits soaks all night and later boiled in 5L of distilled water.
Aqueous extraction is still used for Traditional systems of medicines but it depends on the nature of phytoconstituents of your interest, since the method vary as per the phytoconstituents. So if you provide the nature of phytoconstituents it is easy to modify the method
extraction can be carried out in aqueos media and lyophilised till dryness and can be given in solid form
Depends on what type of phytoconstituents you have in your fruits. If no heat labile constituents are present, boiling would not affect the constituents. If gums and mucilages are present, they may form a fluffy mass on cooling. It largely depends as you can do maceration or percolation or boil them for getting the extract. Regards
Thanks for the responses.
But how about if im not focusing of any phytoconstituents and its a prelimenary study to see the effect towards certain conditions. do i need to freeze dry it or undergo rotary vap? and how about the concentration of the extracts?
freeze drying is recommended over rotary evaporation. At best, the yield of the freeze-dried extracts can be obtained from the mass of the starting material.
The aqueous extract is extracted with solvents of increasing polarity, CHCl3; EtOAc and BuOH. Elimination of the solvent from those extracts produced mixtures of organic compounds that can be analyzed by conventional methods. Exhausted aqueous extract is lyophilized.
Maceration, decoction and cold percolation method may be used for the said purpose.
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