I have stored a stock solution of an amyloid protein in DMSO for a long time. How long it will be stable without aggregating or forming amyloids, so that I can use it for further studies/experiments.
Dear Shreyasi,
this totally depends on your storage conditions (buffer, salt concentration, temperature ...) and needs to be assayed empirically.
Iny any case, you should check on the aggregation state of your amyloid protein using a viable assay. SDS-PAGE (non-denaturing, non-reducing) might help.
A nice and simple assay suitable for your problem might be this:
http://www.ncbi.nlm.nih.gov/pubmed/12711344
Keep in mind that AB is potentially infectious/harmful at exposition!
Best of luck,
Kind regards,
Peter
Hello Shreyasi,
I guess you can also dissolve the amyloid protein in mild basic conditions (eg: Ammonium bicarbonate/Ammonium hydroxide solution) at a high Ph range of 9-10 for storage purposes and for carrying out future experiments. At that hign Ph it will keep the protein stable and prevent aggregation.
Hi mam
Can u tell me specifically as to how many days or months the stock solution can remain stable without aggregating?
Nobody is able to tell you that, since nobody knows about the exact details of the particular specific conditions in your protein sample. How should anyone know the state of your sample? When storing sampkles for a long time, it is best to check on its state via a simple assay.
You will have to figure that out yourself, I'm afraid.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Proteins