How can I prepare disulfiram-copper liposomes using ethanol-based proliposomes method ?

I have a methodology of preparing disulfiram PEGylated liposomes but I am not sure how to attain disulfiram/copper complex in the liposomes.

30 March 2020 4,283 1 View

How can I confirm right colony by PCR?

Hi, I picked up some colonies after electroporation. And I growth those colonies in liquid medium with Erytromycin (200mg/ml). I isolated plasmid after incubation overnight. I did PCR as using...

01 January 2020 9,988 5 View

Significance testing from confidence interval and regression slope?

In a simple regression analysis Seda finds a 95% confidence interval of [-1.4, -0.6] for the unstandardized slope. Is there a statistically significant association between the predictor and the...

07 January 2019 7,050 8 View

SK-MEL 31 cells or B16F10 cells for tyrosinase inhibitor experiments?

Hello Everyone, I have just started to work with animal cell culture studies. I am working on certain novel tyrosinase inhibitors as skin whitening agents. Almost all the literature survey I have...

19 December 2018 8,123 3 View

Why do I see amplification in -RT samples during qPCR?

Hi, During QPCR, I ran non template controls and negative control RT reaction (no RT enzyme using random hexomers and oligodT primers) together with my normal cDNA samples in the same plate. After...

09 July 2018 7,621 4 View

Which steps do you follow after amine synthesis by nitro compounds? and why did the white monomer turn into pink? what can i do not to turn into pink?

02 August 2017 6,211 10 View

Can anyone recommend literature on 'othering'? Is there any socio-pyschological study comparing majority-minority perspectives?

I am specifically asking for socio-psychological studies. I do not like the term "intolerance" since it is politically loaded, but make use of the literature on tolerance, too. I am looking for...

07 September 2015 1,351 6 View

How can I measure the responsivity of a pyroelectric crystal alone?

20 May 2015 5,149 3 View

Which material type is a suitable to design a composite material in ANSYS 14.5?

Thanks in advance for your replies.

28 April 2015 6,558 4 View

Can anyone tell me the lysozyme procedure used for DNA extraction of Lactobacillus sp ?

I need a protocol for lysozyme lysis of Lactobacillus species for DNA extraction from urine and vaginal smear specimens. I have found literatures in which lysozyme lysis is used for Lactobacillus...

11 June 2014 6,886 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?

Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...

03 February 2021 9,311 5 View

What are the factors on which exchange current density of an electrode depends, and how can they be predicted?

While surface roughness is an obvious factor, this is not what is exactly asked. Rather, periodic variation of exchange current density (of an electrode) with solution chemistry (of electrolyte)...

30 January 2021 4,886 5 View

Please how can I justify my regression results?

I conducted a study to investigate stock market reaction to equity issues announcement and i ended up with 64 announcements and i then used the announcement day returns as my DV and investigated...

14 January 2021 1,127 3 View

Can you help with the implementation of two different model of deep learning for stock market prediction?

I have stock price data(Open, high, low, close), I want to do prediction by combining two different models like CNN with others. Dataset will be same, what are the ways that I can use for...

04 January 2021 1,845 3 View

Why does my Giemsa staining solution stay in the filter?

I wanted to use commercial Giemsa staining solution and diluted it 1:10 in pH 6.8 Weise buffer. It says to filter the solution, however, most of the stain seems to preticipate in the filter. The...

04 January 2021 5,028 2 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

03 January 2021 9,947 3 View

Is there central controller for stock market, the Internet, or the food supply of big cities?

One of the properties of complex adaptive systems is inherent order. It means that system can be orderly even without central control. According to Prigogine,1967, Holland,1998, and Kaufman,1995.,...

30 December 2020 2,068 5 View

How to scale up forest carbon stock assessment from plot scale to regional scale?

The research focuses on the mangrove forest ecosystem. Then it will conduct biomass and carbon stock estimation at the plot scale? How to scale up forest carbon stock assessment from plot scale to...

29 December 2020 1,421 3 View