Shreyasi Asthana

7 Questions 15 Answers 0 Followers

Questions related from Shreyasi Asthana

Does Propidium Iodide specifically bind to dead cells?

Hi everyone I am trying to perform live dead assay in a mammalian cell line. I am using DAPi and PI, i.e. ideally DAPI staind nuclear material in all cells and PI must stain nuclear material of...

24 March 2017 518 6 View

How long a stock solution of amyloid proteins is stable in DMSO?

I have stored a stock solution of an amyloid protein in DMSO for a long time. How long it will be stable without aggregating or forming amyloids, so that I can use it for further studies/experiments.

07 June 2016 4,229 4 View

How to calculate miller indicies from TEM SAED image?

I need to calculate hkl values from an SAED image.Using the camera length and the r value I can calculate the d value but how do I assign hkl values.Please help

05 April 2016 6,685 4 View

How would I do MM-PBSA analysis after MD simulations in GROMACS?

how would I do MM-PBSA analysis after MD simulations in GROMACS Is there a tool or software for analysing the MM-PBSA of protein ligand complexes

28 January 2016 5,680 9 View

How might I calculate Guanidinium concentration in molecular dynamics simulation of proteins?

Hi everyone!! While performing the MD simulations on proteins and studying their unfolding by Guanidium, How should one determine the guanidium concentration with regard to the number of molecules...

30 July 2015 4,532 5 View

How do I perform energy minimization of ligands before docking?

Hello everyone I need to dock a protein with some 5 ligands.structures of the four ligands were downloaded from pubchem,where the last one from PDB. I used prodrg server for energy minimization of...

06 May 2015 9,000 18 View

Can anyone help with restriction enzymes?

I have to sub-clone a gene from one vector into another. However I cannot find the correct plasmid with correct MCS. What do I do?

16 April 2014 798 1 View