I tried to isolate full immature gonad from 20-30day fish but failed . plz anyone help me or guide me how to dissect out it .

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Stanley Iheanacho

Anyways, it is dependent on the fish species. However I know that most dissections are done via the ventral part (abdominal cavity) of the fish, slightly above the genital papilia for mainly finishes. Gradual cut is made exposing the gonads and they are carefully detached from the protective sac to avoid puncture of the gonads.

S. M. Banan Khojasteh

This may help you:

https://www.ehp.qld.gov.au/water/monitoring/sampling-manual/pdf/biological-assessment-fish-collection-and-the-dissection-for-the-purpose-of-chemical-analysis-of-tissues.pdf