Fossil leaf-borne fungus recovered from Neogene sediments. Hyphae septate, spores in clusters, with multiple folds.
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Hi fellow researchers, I have got a noob query regarding the question of the post. I am trying to build a data set of enzymes (from UniProt data base) that would contain protein sequences and...
21 April 2024 7,281 4 View
I am trying to infecting huh 7 cell with plasma derived HCV but the infection cannot be established. Has anybody tried infecting any cell line with virus derived from plasma/serum?
03 April 2024 4,685 0 View
As we know, lymph nodes have a mixed population of cells, how do we ensure only B cells are getting isolated and subsequently fused with myeloma cells? Thanks in advance!
30 January 2024 3,449 2 View
Hi, I need help processing for big data. I am trying figure out ''How does Twitter process big data? Also, what can be done with big data?'' Can you explain briefly and/or can you suggest...
05 November 2023 9,122 3 View
Why do ExpiCHO cells need 8% CO2 levels in the incubator? Is is because they have high metabolic rate, thereby producing more lactate and needing more CO2 to buffer the media? Would like to know...
26 August 2023 6,707 3 View
I am facing a problem while running brig with genbank files. I want to give gene annotations & following the manual I have added the genes with the start & stop codon mentioned. But after...
05 August 2023 5,601 0 View
I am facing a problem while running BRIG with genbank files. I have given plasmid sequences as my input files. I want to give gene annotations & following the manual I have added the genes...
05 August 2023 809 0 View
I am facing a problem to circularize a particular bacterial plasmid sequence while doing hybrid assembly in unicycler. After the assembly, it's not showing circular. While the other plasmids have...
29 July 2023 1,251 1 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Dear all, Does anybody have a pdf of this paper? Wood, G.R. and Beeston, J.W., 1986. A late Permian lycopod cone Skilliostrobus sp. cf. S. australis Ash 1979 from Queensland. Geological Survey of...
07 August 2024 4,857 2 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...
26 July 2024 6,520 1 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View
Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...
11 July 2024 6,847 0 View
I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....
11 July 2024 6,251 1 View