What tips to follow while renaming a research paper in your repo for easier and quicker retrieval later during Ph.D research?

We often download tons of research paper related to our Ph.D research domain in their default filename format. After few days, recollecting and finding a particular paper becomes difficult. Any...

12 October 2020 2,425 3 View

What could be the possible reasons for obtaining a precipitate after dissolving fromazan crystals in acidified isopropanol solution in MTT assay?

Hi, I am running an MTT assay where i use an acidic solution of isopropanol, HCl and Triton X -100 to dissolve formazan crystals. I have tried using DMSO before but failed to dissolve crystals...

29 December 2019 6,234 2 View

How do I calculate IC50 of a drug towards OCI AML2 cell line?

Hi, I am in the process of learning to calculate IC50 values of OCI AML2 cells for Cytarabine. I have taken the drug concentrations as 0.01 uM to 50uM. My question is while calculating IC50 is it...

18 December 2019 3,639 3 View

What is the appropriate reagent to dissolve formazan crystals in an MTT assay?

Hi, I am performing an MTT assay on HL60 and OCI AML2 cell lines,to detect the IC50 values for cytarabine. I needed to find the appropriate cell seeding density for which i initially seeded 0.1...

03 December 2019 8,862 3 View

Can someone suggest what type of microscope would be best for imaging human hematopoietic colonies on MethoCult?

I am currently inducing human pluripotent stem cells towards the hematopoietic lineage and assessing the functional capacity of the progenitors by performing colony based assay on MethoCult. I see...

19 February 2019 1,562 3 View

What methods can be used to measure suitability of an ecosystem based on plankton population?

I intend to understand how sustainable an aquatic ecosystem is based on its plankton population. Can anyone help me with suitable methods available?

19 February 2019 3,838 13 View

Would anyone know of a scale that maps paternal involvement or attachment/parenting practices? esp. one that can be used in a non-western context?

03 August 2016 950 6 View

To analyse thin filem using SEM which substrate is preferred? What should be the size and thickness of it?

For forming thin film of metal doped nano TiO2, glass or Silicon surface is preferred and of what size?

26 April 2016 6,656 6 View

How to fine lattice parameters using XRD data?

22 February 2016 5,648 5 View

Can anyone suggest the method of activity measurement for Cystathione-beta synathase (CBS) and Cystathione gama-lyase (CSE) activity in mouse brain?

10 September 2014 6,457 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View