How do I differentiate monocytes into a phenotype similar to Tumor-associated macrophages (for breast cancer)?

More Manasi Kamat's questions See All

How is testing of wound healing and cell attachment properties of novel polymer surfaces done?

I have developed a polymer with active ingredients, I want to test its wound healing and cell attachment capacity to see how it will fare as a wound dressing material. What kind of assay can be...

26 February 2024 7,637 3 View

What precautions have to be taken while synthesizing azo dye consisting a compound with carboxylic acid group?

If one of the starting material has carboxylic acid functional group, how to proceed the reaction?

27 June 2023 951 0 View

Alternative to ZTP models?

Hi all, I was wondering if anyone knew of alternatives to zero - truncated poisson (ZTP) models. I am trying to fit a regression model using demographics on the ZTP. ZTP is doing okay, except the...

27 April 2023 1,574 4 View

What tips to follow while renaming a research paper in your repo for easier and quicker retrieval later during Ph.D research?

We often download tons of research paper related to our Ph.D research domain in their default filename format. After few days, recollecting and finding a particular paper becomes difficult. Any...

12 October 2020 2,607 3 View

Is there any measure or instrument that can be used to assess the students' perceived values of creative tasks?

Specifically, I am looking for a survey measure/ scale/ questionnaire/ inventory of items that focus on the students' perceptions regarding how much they value the creative tasks.

17 May 2020 6,262 0 View

What could be the possible reasons for obtaining a precipitate after dissolving fromazan crystals in acidified isopropanol solution in MTT assay?

Hi, I am running an MTT assay where i use an acidic solution of isopropanol, HCl and Triton X -100 to dissolve formazan crystals. I have tried using DMSO before but failed to dissolve crystals...

29 December 2019 6,323 2 View

How do I calculate IC50 of a drug towards OCI AML2 cell line?

Hi, I am in the process of learning to calculate IC50 values of OCI AML2 cells for Cytarabine. I have taken the drug concentrations as 0.01 uM to 50uM. My question is while calculating IC50 is it...

18 December 2019 3,747 3 View

What is the appropriate reagent to dissolve formazan crystals in an MTT assay?

Hi, I am performing an MTT assay on HL60 and OCI AML2 cell lines,to detect the IC50 values for cytarabine. I needed to find the appropriate cell seeding density for which i initially seeded 0.1...

03 December 2019 8,958 3 View

Drug resistant AML cell lines?

I am working on Acute myeloid leukemia (AML) bone marrow derived Mesenchymal stem cells and will be studying their interactions with AML cells. For standardization process i need to work with drug...

04 November 2019 4,496 5 View

Can someone suggest what type of microscope would be best for imaging human hematopoietic colonies on MethoCult?

I am currently inducing human pluripotent stem cells towards the hematopoietic lineage and assessing the functional capacity of the progenitors by performing colony based assay on MethoCult. I see...

19 February 2019 1,732 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View