Manasi Kamat

3 Questions 3 Answers 0 Followers

Questions related from Manasi Kamat

I need to perform cell migration and invasion assays with monocytes and differentiated macrophages. What are the best protocols?

I have isolated CD14+ monocytes from human PBMC and need to perform migration and invasion assays with these. From my readings, I will be using 5um pore size transwell inserts and 4 hour...

03 May 2017 8,889 5 View

Is the lifespan of tumor associated macrophages known?

I know monocytes survive for around 3-7 days, while macrophages in tissues have a lifespan ranging from months to years. Is anything known about the lifespan of tumor associated macrophages? Thank...

08 November 2016 6,376 1 View

How do I differentiate monocytes into a phenotype similar to Tumor-associated macrophages (for breast cancer)?

Would a good approach be to use supernatants from various breast cancer cell lines and differentiate these CD14+ monocytes from healthy donor blood into macrophages in their presence for 10-14...

01 January 1970 4,894 2 View