Andre Marschall

2 Questions 29 Answers 0 Followers

Questions related from Andre Marschall

Can wrong folding of a membrane transporter protein cause unspecific transport?

I perform recombinant expression of the H+/sucrose symporter SRT1 in yeast, without signal peptide. After the expression, the transporter is purified and reconstituted into proteoliposomes for...

03 August 2015 7,413 9 View

What is the importance of the centrifugation temperature when transferring yeast culture to new medium?

I am working with bog-standard yeast and have to transfer my first culture into an induction medium. The manufacturers protocol for the used vector says to perform this centrifugation and washing...

13 July 2015 1,550 5 View