Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

How is the repeatability interpreted in agreement subject?

Dear Researchers, In a study, we assessed the agreement between two tools. At first, we used Bland-Altman plot. Then we calculated Repeatability Coefficient. Mean Difference (sd): 0.827...

30 January 2021 236 1 View

send me MATLAB code to solve the equations of pumps and signals transactions of Raman amplifier,Please?

Please send me MATLAB code to solve the equations of pumps and signals transactions of Raman amplifier

05 January 2021 283 2 View

Can anyone help with creating treshold variable in tvarx toolbox by gabriel bruneau.?

Dear all, please can anyone help me with how to create the threshold variable array in the Matlab tvarx tool box by Gabriel Bruneau..I have tried all so many ways but I kept getting an error..

01 January 2021 3,757 3 View

How can estimate the Abaqus chaboche parameters from hysteresis curve?

Hello To validate the results of modeling by Abaqus software with experimental results, how can estimate the Chaboche hardening coefficients from the hysteresis curve? The material is steel.

21 December 2020 2,999 3 View

Difference between electro-optical switches and modulators?

Hi Suppose an electro-optical structure that by applying an external voltage, the output light can be in the ON or OFF states. The structure has one input port and one output port. Now, Is this...

10 December 2020 8,276 7 View

What is the reason for the difference in the slope of the hysteresis curve in loading and unloading?

Hello I want to verify a hysteresis curve caused by laboratory results by Abaqus software. The slope of this curve varies in loading and unloading. While the simulation results have the same...

28 November 2020 5,446 9 View

How to calculate of Nonlinear Principal Component Analysis in MATLAB?

We seek to develop an aridity index using nonlinear relationships of climatic variables such as T, P, ET and etc.

23 November 2020 1,660 3 View

Why Hiden mass spectrometer shows wrong percentages after calibration?

Hello every one I was wondering if someone could help me solve the problem that I have with my Mass Spectrometer. Actually I have already calibrated my MS device for a mixture of 60% Ar and 40 %...

19 November 2020 2,984 3 View

Can someone suggest a good reference empirical article linking health outcome with globalization ?

I am trying to see if there's a connection between globalization and health outcome. Going through the KOFGI data set, there are different categories or forms of globalization which includes :...

17 November 2020 2,456 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View