Abdul Rouf

11 Questions 2 Answers 0 Followers

Questions related from Abdul Rouf

How to expand the spheres?

Hello everyone, Greetings! I would like to know if we develop the spheres from secondary cell lines then,how can we expand those developed spheres in order to increase their number for further...

11 November 2019 5,536 2 View

Gene sequences for MACF1 isoforms?

Hello everyone! I am working with Human cancer cell lines. I can't find the gene sequences for MACF1 isoforms(homo sapiens). I just want to design primers for MACF1 isoforms to check the gene...

10 October 2019 5,470 2 View

Designing of primers through uniprot?

Hello everyone, How can a researcher design the primers for a gene of interest using Uniprot.org.A researcher searches the gene of interest in Uniprot.org. in which he gets the gene name alongwith...

10 October 2019 5,714 3 View

How can we develop cancer stem cells from secondary cancer cell lines?

Hello All, I would like to know about the quickest methods as well as reagents to develop cancer stem cells from secondary cancer cell lines. Kindly attach the links for protocols if any. Thanking...

08 August 2019 6,997 3 View

How do the spheroids form in cancer cell lines using B27 supplement?

Hello everyone, I would like to know about the formation of spheroids (for cancer stem cells) in cancer cell lines using B27 supplement. If someone has succeeded in developing spheres in cancer...

05 May 2019 1,788 3 View

Sarcosphere Isolation using centrifugation process?

Hello everyone, I would like to confirm whether we can isolate sarcospheres through centrifugation process or not. If yes, then how much should be the RPM or g, and for how much time....

04 April 2019 3,275 3 View

Is there someone who is working with curcumin currently?

Hello everyone,If someone is working with curcumin at present,then,kindly let me know about the storage process of curcumin stock solution,whether you store the 100mM curcumin stock solution at 4...

04 April 2016 3,433 2 View

Can Curcumin stock be stored for 1 week treatment?

Hi everyone.. I am going to prepare the stock solution of curcumin with DMSO and the working stock with medium and the curcumin treatment is to be continued for 1 week after every 24 hrs.Kindly...

03 March 2016 7,306 4 View

How can we obtain a calibration curve at increasing nanomolar concentrations of Doxorubicin Hydrochloride, using fluorimetry?

Which formula do we have to use in this calculation,either N1V1=N2V2 or something else?

11 November 2015 8,775 1 View

Could someone tell me about different cell based bioassays we can perform using BT474 apart from HER2 ELISA and Cytostat?

So far I have got only cytostatic as well as HER2 ELISA information which we can perform using BT474 Breast cancer cell line.Kindly send me links if u know about few more assays we can perform...

01 January 1970 8,099 2 View

How stop solution i,e; 0.5M H2SO4 halts the reaction?

It is said that 0.5M H2SO4 acts as a stop solution to stop the reaction and turns the green color to yellow.I would like to know how it halts the reaction and how TMB and HRP play their role in...

01 January 1970 6,109 1 View