Hi everyone!
After thinking about it on my own, I'm posting a question here.
Has anyone ever tried creating qPCR standard curve samples (plasmid or DNA standard) by inserting multiple target sequences into a single template and then conducting experiments?
I'm conducting an experiment with four targets and finding it difficult to dilute each plasmid.
I discovered this information on the following site and would like to ask about its feasibility
: https://sg.idtdna.com/pages/education/decoded/article/easily-designed-standard-curves-for-qpcr
Yes, I have done this.
It's a really good way to keep everything standardized.
Pro tip:
Make a very large batch of each concentration, aliquot into "single experiment" volumes in PCR strip tubes, and freeze in your -80*C freezer.
When you set up a qPCR experiment, pull out one set of the standards, thaw, use, and toss the rest.
No worrying about repeated freeze-thaw cycles
Good luck!
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