How to expand the spheres?

Hello everyone, Greetings! I would like to know if we develop the spheres from secondary cell lines then,how can we expand those developed spheres in order to increase their number for further...

10 November 2019 5,441 2 View

Gene sequences for MACF1 isoforms?

Hello everyone! I am working with Human cancer cell lines. I can't find the gene sequences for MACF1 isoforms(homo sapiens). I just want to design primers for MACF1 isoforms to check the gene...

09 October 2019 5,354 2 View

Designing of primers through uniprot?

Hello everyone, How can a researcher design the primers for a gene of interest using Uniprot.org.A researcher searches the gene of interest in Uniprot.org. in which he gets the gene name alongwith...

09 October 2019 5,607 3 View

How can we develop cancer stem cells from secondary cancer cell lines?

Hello All, I would like to know about the quickest methods as well as reagents to develop cancer stem cells from secondary cancer cell lines. Kindly attach the links for protocols if any. Thanking...

07 August 2019 6,890 3 View

How do the spheroids form in cancer cell lines using B27 supplement?

Hello everyone, I would like to know about the formation of spheroids (for cancer stem cells) in cancer cell lines using B27 supplement. If someone has succeeded in developing spheres in cancer...

04 May 2019 1,680 3 View

Sarcosphere Isolation using centrifugation process?

Hello everyone, I would like to confirm whether we can isolate sarcospheres through centrifugation process or not. If yes, then how much should be the RPM or g, and for how much time....

03 April 2019 3,175 3 View

Is there someone who is working with curcumin currently?

03 April 2016 3,329 2 View

Can Curcumin stock be stored for 1 week treatment?

02 March 2016 7,203 4 View

How can we obtain a calibration curve at increasing nanomolar concentrations of Doxorubicin Hydrochloride, using fluorimetry?

Which formula do we have to use in this calculation,either N1V1=N2V2 or something else?

10 November 2015 8,643 1 View

How stop solution i,e; 0.5M H2SO4 halts the reaction?

It is said that 0.5M H2SO4 acts as a stop solution to stop the reaction and turns the green color to yellow.I would like to know how it halts the reaction and how TMB and HRP play their role in...

01 January 1970 6,011 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View