What would be the best survey design and stat. analysis for my research- impact of pandemic on pro-conservation and pro-environmental behaviours?

Ive started to design the survey with demographics, I have a list of pro-environmental and conservation behaviours which i plan to ask if they have changed since the pandemic. I think i'll use...

15 February 2021 4,204 3 View

What company or university lab do you recommend to sequence specific bacterial species from a human microbiome sample?

We are looking for a lab to send human microbiome samples to that can quantify specific bacterial types from . We need a company you have found to be reliable, cost effective and reputable.

07 October 2019 198 4 View

SDS PAGE troubleshooting?

I have been having trouble with SDS PAGE--as you can see there are small bumps in 2nd and 7th (2nd and 8th as well) well. This is precasted gel 4-12%--80V for first 10 min and 120V for 80min. I...

09 September 2019 7,385 8 View

Neuroscience and Computer Science- Coding?

I'd love to hear your opinions/experiences!!! AT ALL LEVELS! What is the best computer program code (to further pursue) for neuroscience models? From chemical/cellular neuroscience to cog...

15 April 2019 3,172 3 View

Mixed Model ANOVA or Multiple Paired Sample T test?

I'm currently working on my research and this is the design: The aim of the research is to investigate the effectiveness of mindfulness on subjective wellbeing and perceived stress. So, 100...

27 July 2018 3,177 3 View

Extracting DNA, how to increase yield from FFPE fatty tissue?

I am extracting DNA and RNA from FFPE tissue, matched colorectal metastasis, primary tumour and matched normal tissue. (RNA is complete, with the DNA I am going on to do exome sequencing and...

02 July 2018 427 3 View

Are there any reagents that work well to directly conjugate fluorophores to antibodies of the IgM isotype?

We are developing a panel of antibodies for flow cytometry but several of the antibodies are of the IgM isotype. We are trying to find a kit or reagent to directly conjugate the antibodies to...

26 June 2018 465 2 View

How can I take images from Olympus fluorescence microscope after saving them on the computer of the microscope?

When I saved the images on the computer of the microscope, they appeared blue for DAPI stained nuclei, and green for FITC stained cellular membranes. When I took the images and opened them on my...

04 January 2018 1,299 3 View

Has anyone figured out a way to avoid condensation on 96 well plates in plate reader?

We are trying to assay bacteria growing at 37 C in plate reader over time and are having condensation problems. Any ideas?

25 April 2017 881 5 View

Has anyone used the 6x6 drop plate method for bacterial enumeration? If so how do you do it so that the 10 ul dots don't spread into each other?

I am trying to do bacterial counts on MRS plates using the drop plate method which uses a dilution series and 10 ul drops on the plate.

27 August 2016 7,024 7 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,351 2 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View

How do I categories into groups on SPSS?

I am using the 'Revised Cyber Bullying Inventory'. This instrument has 2 sub-scales (bully scale and victim scale). From the responses, they will be divided into 4 groups (pure cyberbullies, pure...

13 February 2021 5,787 4 View

Which is the best wavelength to study aerosol optical depth(AOD) related to climate reseach,which criteria must be adopted in choosing the wavelengh?

We are doing a study on impact of aerosol on precipitation. Which satellite data and which wavelength will be the best. or can some one suggest a criteria to be adopted to choose an instrument...

12 February 2021 6,324 3 View