Hello everyone!
I am working with Human cancer cell lines. I can't find the gene sequences for MACF1 isoforms(homo sapiens). I just want to design primers for MACF1 isoforms to check the gene expression in cancer cell lines. I tried to find the gene sequences for these isoforms which I couldnt find in NCBI website. While checking Uniprot.org. I got the Amino Acid sequences for all the 5 isoforms of MACF1 gene.I will be highly indebted if someone could kindly help me get the desired gene sequences to design the primers for all the Macf1 isoforms(homo sapiens)
Thanking you in anticipation..
Hi, you can actually search for any primer sequences from Harvard Primer Bank ( https://pga.mgh.harvard.edu ). All their primers are validated. I have performed a quick search for MACF1 primer and attached in this comment. All the best!
Hi, I like to use www.esembl.org
I have checked there and found the sequences of several protein isoforms for human MACF1. Good luck.
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