I am looking for polymers/biopolymers with high hydrophobicity and high gas permeability. Can any one recommend any types of polymers/biopolymers?

I am looking for polymers/biopolymers with high hydrophobicity and high gas permeability. Can any one recommend any types of polymers/biopolymers?

25 November 2020 5,887 3 View

How to use Incremental/Online learning in face recognition?

I am trying to implement incremental/online learning for a facial recognition application. I've trained a model on a dataset and it works perfectly fine, however, I need to capture new...

23 September 2020 3,819 5 View

How to Fill Up volume in CST MWS?

How to Fill Up volume in CST MWS Hello everybody, Does Anyone know how to fill up a generic volume with a certain material using CST Microwave Studio Software? How to connect a curved plate to...

07 February 2020 4,427 1 View

How to use a CNN model saved as .mat file in python?

Hello everyone, I am going to use a FER2013 fine-tuned pretrained VGG-Face model to extract some features from facial images. Actually, I have the weights and layers of this model saved as a mat...

21 January 2019 4,387 2 View

Which CD20 expressing cell line is better?

Hi there , we are working on antibodies against CD20 antigen ( tositumomab, ofatumumab, Obinutuzumab ) and need a cell line which overexpressing CD20 . as far as I am concerned, Daudi , Ramos...

07 November 2018 1,015 3 View

Can I use SLA1 instead of XHO1?

Hi there. I want to know is it possible to use SLA1 Enz instead of XHO1? My gene of interest is CD20 And I'm working on PSpax, PMD2G plasmid and PCDH. Any suggestion or comment would be...

31 August 2018 7,838 3 View

PCR troubleshooting.what should I do to solve the problem ?

29 August 2018 1,651 6 View

Any suggestion or comment in regard of PCR troubleshooting ?

20 August 2018 150 10 View

Hi.How much blood is needed to gain enough lymphocytes to do RNA extraction?

How many PBMC can be isolated from 10 ml of blood from a healthy donor?

19 July 2018 7,153 3 View

Why does my Blue native gel doesn't run completely?

I have been running blue native gels for my chloroplast samples, the problem is that the gel won't run completely. I have seen gel pictures in different papers that the whole sample runs really...

15 January 2018 4,291 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View