How to expand the spheres?

Hello everyone, Greetings! I would like to know if we develop the spheres from secondary cell lines then,how can we expand those developed spheres in order to increase their number for further...

10 November 2019 5,441 2 View

Gene sequences for MACF1 isoforms?

Hello everyone! I am working with Human cancer cell lines. I can't find the gene sequences for MACF1 isoforms(homo sapiens). I just want to design primers for MACF1 isoforms to check the gene...

09 October 2019 5,354 2 View

Designing of primers through uniprot?

Hello everyone, How can a researcher design the primers for a gene of interest using Uniprot.org.A researcher searches the gene of interest in Uniprot.org. in which he gets the gene name alongwith...

09 October 2019 5,607 3 View

How can we develop cancer stem cells from secondary cancer cell lines?

Hello All, I would like to know about the quickest methods as well as reagents to develop cancer stem cells from secondary cancer cell lines. Kindly attach the links for protocols if any. Thanking...

07 August 2019 6,890 3 View

How do the spheroids form in cancer cell lines using B27 supplement?

Hello everyone, I would like to know about the formation of spheroids (for cancer stem cells) in cancer cell lines using B27 supplement. If someone has succeeded in developing spheres in cancer...

04 May 2019 1,680 3 View

Is there someone who is working with curcumin currently?

03 April 2016 3,329 2 View

Can Curcumin stock be stored for 1 week treatment?

02 March 2016 7,203 4 View

How can we obtain a calibration curve at increasing nanomolar concentrations of Doxorubicin Hydrochloride, using fluorimetry?

Which formula do we have to use in this calculation,either N1V1=N2V2 or something else?

10 November 2015 8,643 1 View

Could someone tell me about different cell based bioassays we can perform using BT474 apart from HER2 ELISA and Cytostat?

01 January 1970 8,004 2 View

How stop solution i,e; 0.5M H2SO4 halts the reaction?

It is said that 0.5M H2SO4 acts as a stop solution to stop the reaction and turns the green color to yellow.I would like to know how it halts the reaction and how TMB and HRP play their role in...

01 January 1970 6,011 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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23 February 2021 4,014 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View