Gertrudis Rojas

4 Questions 402 Answers 0 Followers

Questions related from Gertrudis Rojas

How can I transfect HEK-293 cells in suspension without Freestyle medium?

Hi We were transfecting HEK-293 cells adapted to grow in suspension to produce recombinant proteins with excellent results. Cells were maintained in Freestyle, transfected with PEI/DNA also in...

11 November 2019 8,047 3 View

Can I use the Qiaprep 96 Turbo kit with a microplate centrifuge instead of vacuum?

I got from someone else Qiaprep 96 Turbo kits for high throuhghput plasmid DNA purification. But I dont have QIAVAC96 or a similar device to pass the solutions using vauum. Can I do it using a...

08 August 2018 1,760 0 View

How can I compare ELISA and BIAcore results?

Higher affinities result in higher ELISA signals, of course. But now Im working with several mutated ligands and their receptor. The wt ligand has an affinity to the receptor around 20nM. Several...

09 September 2015 7,035 8 View

Has anyone tried to obtain recombinant IL-2 in HEK293 cells?

Can you provide plasmid details and the protocol?

08 August 2015 7,717 2 View