Hi
i am trying to grow Arabidopsis thaliana plants in MS agar plates ( vertically). But everytime i am getting too much condensation in my dishes. (square dishes ). though i sealed the dishes with parafilm, but it didn't help. Can anyone suggest me, how to get rid of it?
Thank you
Hi Swati,
Please refer to this similar discussion on RG:
How do you get rid of condensation from petriplates regenerating rice calli?
https://www.researchgate.net/post/How_do_you_get_rid_of_condensation_from_petriplates_regenerating_rice_calli/1
after pouring the agar plates, leave the lid slightly open in a sterile hood till the plates cool down. It may take about a hour to be safe. Then leave put the lids fully and leave it at room temperature overnight.
Instead of using parafilm, for many years we have been using medical gas-permeable tape (Micropore™ Surgical Tape; 3M Health Care, USA) [see below] to wrap the plates, which reduces the condensation in plates.
vapour goes higher. stacking is good because of mjnjmalisation of heat gradient. think it over, it is simple.turning is good again, vapour will goes onto agar.
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