I want to do SPME-GC. Can anyone suggest me the right way?

More Swati Tyagi's questions See All

How to draw graphs using circos ?

Hello Mates I have 23 chloroplast genome data and want to plot a comparative graph . In comparatives genomic studies, i have seen people use Circos to make some easy representation. Can someone...

02 March 2019 9,051 3 View

Weird Melt curve in qPCR. can someone help??

I run qPCR with cDNA and got nice amplification curve but melt curve is really horrible. I extracted RNA from bacteria using Trizol and digest RNA with DNase and synthesized cDNA with Takara 1st...

31 December 2017 8,020 5 View

Can I use ITS region of fungi as endogenous control for RT-PCR?

I treated my fungal samples with Chemical controlling agent that almost killed the fungal pathogen. In treated condition, No other house keeping gene is expressed except the ITS. I wonder if i can...

07 August 2017 630 24 View

How can i use flow cytometer for detecting fungal (sclerotinia sclerotiorum )cell membrane integrity ?

I want to check the cell membrane integrity of my fungal samples treated with chemical agent. i heard using flow cytometery but my sample is filamentous fungi (that can block the filters). Does...

07 August 2017 1,493 0 View

Can anyone provide me with Polymyxa betae infected roots?

I am working on Plasmodiophora and to further explore my study i want polymyxa beate infected roots. I wonder if there anyone who can provide me. Thanks

08 September 2016 8,236 5 View

How can I get rid of condensation in MS agar plates?

Hi i am trying to grow Arabidopsis thaliana plants in MS agar plates ( vertically). But everytime i am getting too much condensation in my dishes. (square dishes ). though i sealed the dishes...

06 July 2015 5,530 6 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

What can i do to find the right topic for my thesis?

i am facing the difficulty to find the right and latest topic for my thesis. i searched and read hundreds of articles but i couldn't find the right one. what can i do I'm really confused.

02 August 2024 1,323 8 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 180 3 View