How to draw graphs using circos ?

Hello Mates I have 23 chloroplast genome data and want to plot a comparative graph . In comparatives genomic studies, i have seen people use Circos to make some easy representation. Can someone...

02 March 2019 8,957 3 View

Weird Melt curve in qPCR. can someone help??

I run qPCR with cDNA and got nice amplification curve but melt curve is really horrible. I extracted RNA from bacteria using Trizol and digest RNA with DNase and synthesized cDNA with Takara 1st...

31 December 2017 7,874 5 View

Can I use ITS region of fungi as endogenous control for RT-PCR?

I treated my fungal samples with Chemical controlling agent that almost killed the fungal pathogen. In treated condition, No other house keeping gene is expressed except the ITS. I wonder if i can...

07 August 2017 504 24 View

How can i use flow cytometer for detecting fungal (sclerotinia sclerotiorum )cell membrane integrity ?

I want to check the cell membrane integrity of my fungal samples treated with chemical agent. i heard using flow cytometery but my sample is filamentous fungi (that can block the filters). Does...

07 August 2017 1,387 0 View

Can anyone provide me with Polymyxa betae infected roots?

08 September 2016 8,145 5 View

How can I get rid of condensation in MS agar plates?

06 July 2015 5,428 6 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View