How to draw graphs using circos ?

Hello Mates I have 23 chloroplast genome data and want to plot a comparative graph . In comparatives genomic studies, i have seen people use Circos to make some easy representation. Can someone...

02 March 2019 8,957 3 View

Weird Melt curve in qPCR. can someone help??

I run qPCR with cDNA and got nice amplification curve but melt curve is really horrible. I extracted RNA from bacteria using Trizol and digest RNA with DNase and synthesized cDNA with Takara 1st...

31 December 2017 7,874 5 View

Can I use ITS region of fungi as endogenous control for RT-PCR?

I treated my fungal samples with Chemical controlling agent that almost killed the fungal pathogen. In treated condition, No other house keeping gene is expressed except the ITS. I wonder if i can...

07 August 2017 504 24 View

Can anyone provide me with Polymyxa betae infected roots?

08 September 2016 8,145 5 View

I want to do SPME-GC. Can anyone suggest me the right way?

08 September 2016 2,742 2 View

How can I get rid of condensation in MS agar plates?

06 July 2015 5,428 6 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

No title

02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View