Swati Tyagi

7 Questions 17 Answers 0 Followers

Questions related from Swati Tyagi

How to draw graphs using circos ?

Hello Mates I have 23 chloroplast genome data and want to plot a comparative graph . In comparatives genomic studies, i have seen people use Circos to make some easy representation. Can someone...

03 March 2019 9,023 3 View

Weird Melt curve in qPCR. can someone help??

I run qPCR with cDNA and got nice amplification curve but melt curve is really horrible. I extracted RNA from bacteria using Trizol and digest RNA with DNase and synthesized cDNA with Takara 1st...

01 January 2018 7,992 5 View

Can I use ITS region of fungi as endogenous control for RT-PCR?

I treated my fungal samples with Chemical controlling agent that almost killed the fungal pathogen. In treated condition, No other house keeping gene is expressed except the ITS. I wonder if i can...

08 August 2017 589 24 View

How can i use flow cytometer for detecting fungal (sclerotinia sclerotiorum )cell membrane integrity ?

I want to check the cell membrane integrity of my fungal samples treated with chemical agent. i heard using flow cytometery but my sample is filamentous fungi (that can block the filters). Does...

08 August 2017 1,462 0 View

Can anyone provide me with Polymyxa betae infected roots?

I am working on Plasmodiophora and to further explore my study i want polymyxa beate infected roots. I wonder if there anyone who can provide me. Thanks

09 September 2016 8,208 5 View

I want to do SPME-GC. Can anyone suggest me the right way?

i am working with Volatile organic compounds produced by Bacteria and Fungi. Want to test it by SPME-GC. Can anyone suggest how to prepare the sample and analyse it? Thanks

09 September 2016 2,807 2 View

How can I get rid of condensation in MS agar plates?

Hi i am trying to grow Arabidopsis thaliana plants in MS agar plates ( vertically). But everytime i am getting too much condensation in my dishes. (square dishes ). though i sealed the dishes...

07 July 2015 5,504 6 View