How to draw graphs using circos ?

Hello Mates I have 23 chloroplast genome data and want to plot a comparative graph . In comparatives genomic studies, i have seen people use Circos to make some easy representation. Can someone...

02 March 2019 8,957 3 View

Weird Melt curve in qPCR. can someone help??

I run qPCR with cDNA and got nice amplification curve but melt curve is really horrible. I extracted RNA from bacteria using Trizol and digest RNA with DNase and synthesized cDNA with Takara 1st...

31 December 2017 7,874 5 View

Can I use ITS region of fungi as endogenous control for RT-PCR?

I treated my fungal samples with Chemical controlling agent that almost killed the fungal pathogen. In treated condition, No other house keeping gene is expressed except the ITS. I wonder if i can...

07 August 2017 504 24 View

How can i use flow cytometer for detecting fungal (sclerotinia sclerotiorum )cell membrane integrity ?

I want to check the cell membrane integrity of my fungal samples treated with chemical agent. i heard using flow cytometery but my sample is filamentous fungi (that can block the filters). Does...

07 August 2017 1,387 0 View

I want to do SPME-GC. Can anyone suggest me the right way?

08 September 2016 2,742 2 View

How can I get rid of condensation in MS agar plates?

06 July 2015 5,428 6 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View