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Questions related from Bhaskar Chatterjee
We usually can determine LNP encapsulation efficiency (though indirectly) by Ribogreen assay. What can be the best way to determine encapsulation efficiency of polyplex micelles?
17 July 2025 7,954 2 View
Suppose I need to target mitochondria to address malignancy-related problems. Are there peptide-based ligands similar to Nuclear Localization Signals (NLS) for nuclear targeting that can be used...
21 June 2024 9,674 1 View
To culture the U-87-MG cell line, various media formulations are available online. These include: a. Essential Minimum Eagle Medium (EMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL...
08 January 2024 667 0 View
For the past few weeks, I've been attempting to passage U-87-MG cells in our lab. However, after thawing, instead of reaching 80% confluency within 24 hours, they're taking 3-4 days to achieve the...
22 December 2023 573 2 View
I am currently doing a project on phytochemicals and its anti-cancer properties. My question is actually in two parts: I have a ligand molecule ( a dietary phytochemical) and I am studying its...
22 March 2021 8,677 5 View
A particular gene (gene A) is reported to be downregulated in cancer cells as compared to the normal cells. How can you prove this with the help of PCR?
28 September 2020 5,678 4 View
in Gastric cancer both cancer stem cells and tumor microenvironment prevents the action of therapeutics and drugs. a. How can I find a co-relation between them? b. How can i evaluate their role...
07 May 2020 6,668 7 View
Is there a distinction between mRNA engineering and mRNA bundling in the context of nanomedicine, or does one fall under the other as a subtype? How can we discern this context and understand the...
01 January 1970 3,904 0 View
I regularly perform qPCR and ELISA, almost every week, and the number of samples is gradually increasing over time. I was recently informed that using R instead of Excel could simplify the...
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