I will use that to treat fibroblast cell culture.
Is it palmitate (as sodium palmitate) or as the free palmitatic acid? What concentration do you need?
Sodium palmitate is soluble in water; the free acid is much less polar ( see http://pubs.acs.org/doi/pdf/10.1021/je8005979 )
It is sodium palmitate, but it is not dissolved in water, we tried everything. We need almost 5mM.
Hi there, for my experiments I used the protocol described in this paper (free in PubMed central):
Coleman DT, Bigelow R, Cardelli JA - Mol Cancer Ther January 2009 8;214
Making the solution is a bit tricky you need to keep the solution stirring at 50°C.
Hope it will work for you and let me know if you need other help.
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