Hi, I was trying to remove sialic acid from Hela cell surface using neuraminidase -NA- ( Vibrio cholerae ). I firstly fixed cells with PFA and treated them with 200mU/ml NA for 1 hour at 37C. To see the removal of the sialic acid I used WGA ( Wheat germ agglutinin a king of lectin) labeled with FITC. What I was planning to see is that if the neuraminidase remove the sialic acid, WGA cannot bind and I see less FITC under the microscope. However, everytime I see nearly the same amount FITC for both non-treated and NA treated samples.
I am wondering if I wasn't able to remove sialic acid with NA or I was not able to see the removal with using lectin binding assay?
Daniel Beckers
Hi Cagla,
Just curious if you were able to resolve this at all?
Thanks!
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