Why I cannot remove sialic acid from cell surface?

Hi, I was trying to remove sialic acid from Hela cell surface using neuraminidase -NA- ( Vibrio cholerae ). I firstly fixed cells with PFA and treated them with 200mU/ml NA for 1 hour at 37C. To...

29 May 2020 5,663 1 View

How can I prepare disulfiram-copper liposomes using ethanol-based proliposomes method ?

I have a methodology of preparing disulfiram PEGylated liposomes but I am not sure how to attain disulfiram/copper complex in the liposomes.

30 March 2020 4,283 1 View

How can I confirm right colony by PCR?

Hi, I picked up some colonies after electroporation. And I growth those colonies in liquid medium with Erytromycin (200mg/ml). I isolated plasmid after incubation overnight. I did PCR as using...

01 January 2020 9,988 5 View

Significance testing from confidence interval and regression slope?

In a simple regression analysis Seda finds a 95% confidence interval of [-1.4, -0.6] for the unstandardized slope. Is there a statistically significant association between the predictor and the...

07 January 2019 7,050 8 View

SK-MEL 31 cells or B16F10 cells for tyrosinase inhibitor experiments?

Hello Everyone, I have just started to work with animal cell culture studies. I am working on certain novel tyrosinase inhibitors as skin whitening agents. Almost all the literature survey I have...

19 December 2018 8,123 3 View

Why do I see amplification in -RT samples during qPCR?

Hi, During QPCR, I ran non template controls and negative control RT reaction (no RT enzyme using random hexomers and oligodT primers) together with my normal cDNA samples in the same plate. After...

09 July 2018 7,621 4 View

Is there a database that shows HOMO/LUMO levels or valence/conduction bands of the materials?

Hello I want to figure out if I can find the energy levels of the materials from a specific source for research purpose. Or should I extract these levels from various articles? The same question...

22 February 2018 1,286 2 View

Which steps do you follow after amine synthesis by nitro compounds? and why did the white monomer turn into pink? what can i do not to turn into pink?

02 August 2017 6,211 10 View

How can I dissolve and store sodium palmitate, HPLC grade water and DMSO do not work. Is there any other way?

I will use that to treat fibroblast cell culture.

12 June 2016 4,200 3 View

Can anyone recommend literature on 'othering'? Is there any socio-pyschological study comparing majority-minority perspectives?

I am specifically asking for socio-psychological studies. I do not like the term "intolerance" since it is politically loaded, but make use of the literature on tolerance, too. I am looking for...

07 September 2015 1,351 6 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

Can Virtual Servers (remote dekstop manager) run heavy ANSYS Fluent CFD simulation ?

I have a virtual machine. My host Pc specs are not that good thats why i went for virtual servers. so basically my host pc has 8gb of ram with nvidia quadro k2200 4Gb DDR5 GPU ,processor intel...

01 March 2021 8,415 1 View

How to increase mesh quality of a cfd model in ansys fluent?

How can one improve mesh skewness, orthogonality, aspect ratio?

28 February 2021 7,730 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View