Why I cannot remove sialic acid from cell surface?

Hi, I was trying to remove sialic acid from Hela cell surface using neuraminidase -NA- ( Vibrio cholerae ). I firstly fixed cells with PFA and treated them with 200mU/ml NA for 1 hour at 37C. To...

29 May 2020 5,663 1 View

Do you know any blotch detection dataset for image reconstruction?

10 October 2018 546 3 View

Is there a database that shows HOMO/LUMO levels or valence/conduction bands of the materials?

Hello I want to figure out if I can find the energy levels of the materials from a specific source for research purpose. Or should I extract these levels from various articles? The same question...

22 February 2018 1,286 2 View

No title

29 October 2017 7,531 9 View

Does anyone used T1063 Thrombin, how should it be solved?

Hello, I will prepare fibrin gel from this product (T1063/250 UN), and need to solve this product in 50 UI/mL concentration. Please can anyone describe: how should it be calculated and diluted to...

24 October 2017 9,348 3 View

How can I dissolve and store sodium palmitate, HPLC grade water and DMSO do not work. Is there any other way?

I will use that to treat fibroblast cell culture.

12 June 2016 4,200 3 View

How can I plot roc curve for multiclass classification by using knn?

25 April 2016 545 2 View

What type of technical question may be asked in a technical interview of computer science for the post of a lecturer?

29 December 2015 1,846 4 View

Which material type is a suitable to design a composite material in ANSYS 14.5?

Thanks in advance for your replies.

28 April 2015 6,558 4 View

No title

06 January 2015 296 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View