PDX leukemia in immunodeficient mice show regression after a time. Anyone knows what is going on?

I am performing PDX ALL in NSGS mice, but something curious happens: some leukemias develop very well , but after a time the hCD45+ cells show a huge regression while the mCD45+ cells turn back to...

16 December 2020 7,344 2 View

About the mouse humanization process, why should I wait between four to 24 hours after irradiation to transplant the stem cells into pups?

The protocol for the humanization of the NSG mice with human CD34+ cells demands that mice should be irradiated before the inoculation of the human cells. However, after this procedure, it is...

01 September 2020 8,477 4 View

How to calculate dielectric constant from oil spill in SAR satellite image?

I'm trying to understand how to determine relationship between reflectivity in SAR images and dieletric constant of oil spill offshore registred in image.

17 August 2020 2,094 2 View

What is the best methodology to identify and to quantify different classes of lipids from pollen?

I would like to characterize and quantify different classes of lipids of different samples of pollen. I was thinking to use HPLC or GC-MS. Someone know if these techniques are good for that?...

09 April 2018 3,379 4 View

Xenograft of DND-41 cell line in NUDE mice.

I am trying to develop subcutaneous xenograft model in NUDE mice, using DND-41 tumor cells. I already tried sometimes, without success. I am using 100% and/or 50% of matrigel.Someone knows if is...

22 February 2016 7,994 8 View

There is some special condition for culturing BT474 breast cancer cell line?

I am used to work with BT474 cell line. But as I was needing a huge amount of cells, I expanded them in 4 flask T150 during 3 weeks. It is known that the growth is very slow, but suddenly, when...

22 February 2016 2,431 5 View

Do you know any software that can analyze absence/presence dataset from allopolyploid microsatellite data?

12 October 2015 1,282 4 View

Can I supplement the culture medium with mouse serum instead of bovine serum to culture the cells?

13 February 2014 4,745 2 View

How to determine an antibody titer in a Whole Cell ELISA?

I am trying to get a monoclonal antibody against a single transmembrane protein. I am using the Whole Cell ELISA to test the immunized mouse serum. The serum is tested against the cell with or...

02 September 2013 2,649 13 View

How obtain stable transfect Hek293T cell line using blasticidin to selection?

I tried to stably transfect Hek293T with linearized pEF6/V5 plasmid, that contain the blasticidin resistance gene. The negative control cells (no-transfected Hek293T) died into about one week. I...

04 October 2012 214 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View