Encyclopedia of Animal Cognition and Behavior
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I would like to simulate a congestion scenario using SUMO-TraCi interface. I was able to create the network layout and a few cars. How to generate an increasing number of vehicles moving at...
20 November 2023 1,907 1 View
I seeded PBMCs in a 10 cm petri dish (1x10^8) and treated them with hM-CSF and incubated them at 37C with 5% CO2 for 3 days. After 3 days, I carefully added PBS to wash and lost most of my cells....
12 November 2023 2,523 5 View
Error [65]: Error, extent of vector too large or attribute table error." I uninstalled and reinstalled QGIS 3.26.2 but I still get the error. Any idea what is causing this?
07 August 2023 4,797 1 View
As a final project for a college course, we need to do a primary culture of non parenquimatic liver cells from mice. In our first try we obtained a great amount of white blood cells instead of...
14 May 2023 9,576 2 View
I want to grow (in vitro) primary cells from different types of pediatric tumors. It is known that fetal bovine serum induces cell differentiation in embryonic tumors, mainly. What is the most...
21 September 2022 1,040 0 View
We are used the protocol for urease extration adaptated (to C. neoformans) from Amin et al.,2013: Briefly, the broth cultures were subjected to centrifugation (5,000 × g, 4 °C) and the...
25 November 2021 4,404 2 View
In order to produce a chimeric antibody, I transformed HEK293T cells with two plasmids: one for heavy chain and the other for light chain IgG. The supernatant was purified in a Protein G-Sepharose...
28 March 2021 4,711 8 View
I am performing PDX ALL in NSGS mice, but something curious happens: some leukemias develop very well , but after a time the hCD45+ cells show a huge regression while the mCD45+ cells turn back to...
16 December 2020 7,505 2 View
The protocol for the humanization of the NSG mice with human CD34+ cells demands that mice should be irradiated before the inoculation of the human cells. However, after this procedure, it is...
01 September 2020 8,630 4 View
I'm trying to understand how to determine relationship between reflectivity in SAR images and dieletric constant of oil spill offshore registred in image.
17 August 2020 2,292 2 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
Hello everyone I am looking for one or some books for propertes and behaviors of stochastic fibre orientation composites. unfotunately I could not find any suitable reference for thias by...
04 August 2024 3,461 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I am editing a book for Springer Nature on recent advancements and future trends in biomaterials for orthopedic and trauma applications, with a publication date slated for early next year. If you...
29 July 2024 5,015 9 View
Some educational books with practical exercises of the "Idrisi Selva" software, which have step-by-step visual training for predicting the change of land use/land cover and including the use of...
28 July 2024 4,168 0 View
Several Complex Variables and Integral Formulas Dear researchers, how can we download this book? Please help?
22 July 2024 4,120 4 View
How to determine tannin content in fodder pellets?
22 July 2024 8,163 3 View
In the aquaculture and fish nutrition research field, a number of growth and somatic indexes are often used, including specific growth rate (SGR), feed conversion rate (FCR), protein efficiency...
22 July 2024 2,480 4 View
I'm trying to use cassowary footprint to estimate their population since their footprints are distinguishable from other animal on the field. is it really possible to do that? since it's really...
18 July 2024 3,729 7 View