PDX leukemia in immunodeficient mice show regression after a time. Anyone knows what is going on?

I am performing PDX ALL in NSGS mice, but something curious happens: some leukemias develop very well , but after a time the hCD45+ cells show a huge regression while the mCD45+ cells turn back to...

16 December 2020 7,344 2 View

About the mouse humanization process, why should I wait between four to 24 hours after irradiation to transplant the stem cells into pups?

The protocol for the humanization of the NSG mice with human CD34+ cells demands that mice should be irradiated before the inoculation of the human cells. However, after this procedure, it is...

01 September 2020 8,477 4 View

How to calculate dielectric constant from oil spill in SAR satellite image?

I'm trying to understand how to determine relationship between reflectivity in SAR images and dieletric constant of oil spill offshore registred in image.

17 August 2020 2,094 2 View

What is the best methodology to identify and to quantify different classes of lipids from pollen?

I would like to characterize and quantify different classes of lipids of different samples of pollen. I was thinking to use HPLC or GC-MS. Someone know if these techniques are good for that?...

09 April 2018 3,379 4 View

What is the future of labor law in a future world without work?

Labor law is predominantly carried out from the domestic perspective of the countries through their legislation, accepting only some international (often disregarded) legislative...

23 March 2018 473 6 View

Xenograft of DND-41 cell line in NUDE mice.

I am trying to develop subcutaneous xenograft model in NUDE mice, using DND-41 tumor cells. I already tried sometimes, without success. I am using 100% and/or 50% of matrigel.Someone knows if is...

22 February 2016 7,994 8 View

There is some special condition for culturing BT474 breast cancer cell line?

I am used to work with BT474 cell line. But as I was needing a huge amount of cells, I expanded them in 4 flask T150 during 3 weeks. It is known that the growth is very slow, but suddenly, when...

22 February 2016 2,431 5 View

Do you know any software that can analyze absence/presence dataset from allopolyploid microsatellite data?

12 October 2015 1,282 4 View

Can I supplement the culture medium with mouse serum instead of bovine serum to culture the cells?

13 February 2014 4,745 2 View

Can you suggest a good assay to determine paracellular permeability in epithelial cells?

I am establishing a bovine oviductal epithelial cells culture using inserts of 0.4um pores and I am trying to get the cells polarized with this system. I would like to use the permeability assay...

16 January 2014 7,820 18 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View