15 Questions 21 Answers 0 Followers
Questions related from Priscila Zenatti
I want to grow (in vitro) primary cells from different types of pediatric tumors. It is known that fetal bovine serum induces cell differentiation in embryonic tumors, mainly. What is the most...
22 September 2022 1,011 0 View
In order to produce a chimeric antibody, I transformed HEK293T cells with two plasmids: one for heavy chain and the other for light chain IgG. The supernatant was purified in a Protein G-Sepharose...
29 March 2021 4,674 8 View
I am performing PDX ALL in NSGS mice, but something curious happens: some leukemias develop very well , but after a time the hCD45+ cells show a huge regression while the mCD45+ cells turn back to...
17 December 2020 7,453 2 View
The protocol for the humanization of the NSG mice with human CD34+ cells demands that mice should be irradiated before the inoculation of the human cells. However, after this procedure, it is...
02 September 2020 8,587 4 View
I have cultivated some hybridoma clones for about two months, since I thaw them. I have tested for antibody production by Flow Cytometry and ELISA gathering positive results. Then, I expanded them...
20 March 2019 1,742 4 View
To test in vitro toxicity effect of monoclonal antibodies (from hybridoma cells), I want to perform CDC and ADCC assay. I want to use the bovine or human serum as Complement Proteins source. I do...
05 February 2019 7,903 2 View
I am trying to develop subcutaneous xenograft model in NUDE mice, using DND-41 tumor cells. I already tried sometimes, without success. I am using 100% and/or 50% of matrigel.Someone knows if is...
23 February 2016 8,103 8 View
I am used to work with BT474 cell line. But as I was needing a huge amount of cells, I expanded them in 4 flask T150 during 3 weeks. It is known that the growth is very slow, but suddenly, when...
23 February 2016 2,527 5 View
I am trying to culture murine cell line (lymphocytes) in medium supplemented with serum of mouse bought from Sigma (cat# M5905). I inactivated the serum in water bath at 56°C for 1hour. Next I...
14 February 2014 4,831 2 View
I am trying to get a monoclonal antibody against a single transmembrane protein. I am using the Whole Cell ELISA to test the immunized mouse serum. The serum is tested against the cell with or...
03 September 2013 2,884 13 View
I tried to stably transfect Hek293T with linearized pEF6/V5 plasmid, that contain the blasticidin resistance gene. The negative control cells (no-transfected Hek293T) died into about one week. I...
05 October 2012 386 3 View
I've sorted 293T cells for GFP positive (remember this is an adherent cell line). I used BD FACS Aria Ilu. The cells were very beautiful before the sorting and I got 92% of positive cells. Cells...
02 October 2012 5,907 24 View
I need to check protein expression at the cell membrane. I've used a PE-conjugated antibody but the cells express constitutively GFP and I can't compensate it. Does anyone know another color I can...
27 September 2012 1,122 32 View
I have doubts about how many cells I can seed in different wells or plates. I would like to know some tips for determining this or to know a site that I can find this information.
16 July 2012 3,723 20 View
I got some frozen cell that came from USA to Brazil. At the thaw the cells looks like so good, but in about 24/48 hours they are completely dead. The cells need growth factor to survive and it was...
13 July 2012 3,310 5 View
