The protocol for the humanization of the NSG mice with human CD34+ cells demands that mice should be irradiated before the inoculation of the human cells. However, after this procedure, it is mandatory to wait at least 4h, but no more than 24h, to perform the inoculation of the stem cells. I do not know why it is not recommended to inject the cells immediately after irradiation. If someone has experience with this methodology, please, let me know. Thanks.
Myeloablation results in severe suppression of the immune system. Immediately post-irradiation mice feel very stress, therefore, we must wait for mice to settle after irradiation. We injected huCD34 cells to mice after 18-20 hr post-irradiation and successfully generated humanized mice. Our research article is now in the peer-review process. I may share it after publication.
Dear Kamal Pandey, thank you for your answer. I would like to have a copy of your article when it is accepted for publish.
Do you use huCD34 cell purified or do you use the whole blood lymphocytes?
Dear Priscila, Yes I definitely will provide you a copy of our article after acceptance. Yes, we isolated huCD34 using MACS, Confirmed the CD34+ cells via FACS and injected to mice.
Thank you, Kamal. I am looking forward to have a copy of your article.
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