PDX leukemia in immunodeficient mice show regression after a time. Anyone knows what is going on?

I am performing PDX ALL in NSGS mice, but something curious happens: some leukemias develop very well , but after a time the hCD45+ cells show a huge regression while the mCD45+ cells turn back to...

16 December 2020 7,344 2 View

About the mouse humanization process, why should I wait between four to 24 hours after irradiation to transplant the stem cells into pups?

The protocol for the humanization of the NSG mice with human CD34+ cells demands that mice should be irradiated before the inoculation of the human cells. However, after this procedure, it is...

01 September 2020 8,477 4 View

How to calculate dielectric constant from oil spill in SAR satellite image?

I'm trying to understand how to determine relationship between reflectivity in SAR images and dieletric constant of oil spill offshore registred in image.

17 August 2020 2,094 2 View

What is the best methodology to identify and to quantify different classes of lipids from pollen?

I would like to characterize and quantify different classes of lipids of different samples of pollen. I was thinking to use HPLC or GC-MS. Someone know if these techniques are good for that?...

09 April 2018 3,379 4 View

Xenograft of DND-41 cell line in NUDE mice.

I am trying to develop subcutaneous xenograft model in NUDE mice, using DND-41 tumor cells. I already tried sometimes, without success. I am using 100% and/or 50% of matrigel.Someone knows if is...

22 February 2016 7,994 8 View

There is some special condition for culturing BT474 breast cancer cell line?

I am used to work with BT474 cell line. But as I was needing a huge amount of cells, I expanded them in 4 flask T150 during 3 weeks. It is known that the growth is very slow, but suddenly, when...

22 February 2016 2,431 5 View

Do you know any software that can analyze absence/presence dataset from allopolyploid microsatellite data?

12 October 2015 1,282 4 View

Can I supplement the culture medium with mouse serum instead of bovine serum to culture the cells?

13 February 2014 4,745 2 View

How to determine an antibody titer in a Whole Cell ELISA?

I am trying to get a monoclonal antibody against a single transmembrane protein. I am using the Whole Cell ELISA to test the immunized mouse serum. The serum is tested against the cell with or...

02 September 2013 2,649 13 View

How obtain stable transfect Hek293T cell line using blasticidin to selection?

I tried to stably transfect Hek293T with linearized pEF6/V5 plasmid, that contain the blasticidin resistance gene. The negative control cells (no-transfected Hek293T) died into about one week. I...

04 October 2012 214 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View