Hi, I need to produce HBe Ag would ney one please know what cell lines are the best to work for HBe Ag prosuction and any one have the method or research citation as well. please mentor me
I made 400 L of MEM 199 medium (sigma powder cat M0393 Medium 199 ) and added extra 8 L of water. Some people saying that i over diluted this media and it wont be good. Please give your opinion....
09 October 2016 2,319 3 View
Why and what type of cell lines we use these type of trypsin 1. Trypsin 0.25% vs 2. Trypsin /EDTA 0.25% 1mM EDTA 3. types of cells line we use above mentioned trypsins
03 April 2016 5,508 4 View
I am trying to bind porcine saliva (2.3 mg/ml) on MaxiSorp plate. Any one have idea how to detect that protein really bind in the well. Please recommend me a method that can detect the...
08 September 2015 4,039 1 View
I am trying to do flow cytometry analysisto observe the effect of LPS and CPG on DC maturation (immature vs mature DC's) on pig tonsil tissue. What flow cytometry markers (ab) do I need and how...
02 March 2015 6,118 2 View
Has anyone ever tried to amplify or perform qPCR on UV treated (80mj) RNA of Rubella Virus?
31 December 2013 7,957 0 View
QPCR to UV treated rubella RNA.
11 December 2013 3,983 0 View
.
01 February 2013 1,104 1 View
Hi, I am looking for some key reserch article to produce IPNA Hemorrhagic virus from Salmon base cell line. Can any one help me please. thanks
09 October 2010 5,347 0 View
I need to How PERFULOROPOLY ETHER works to seprate proteins?
03 April 2010 9,685 0 View
Hi, Would any one answer my querry. why we washes hollow fiber membrane filter with 0.1 M NaOH and 0.5 NaOH after virus concentration is done?. thank
02 March 2010 5,881 0 View
Hello all. I grew ARPE-19 cells in cell culture and infected them with a virus (Varicella-zoster virus). After the virus reached a high infection rate, I harvested everything in the plate and use...
07 May 2024 259 3 View
Hi follow virologists, I'm new to the virology field and I'm eager to understand how freeze/thaw cycles affect virus culture fluid titers. I've noticed that while some viruses show increased...
18 March 2024 5,174 4 View
Dear virologists; What is the PCR technique used in virology to detect viral nucleic acids? The steps involved. I'd also like to know, since some viruses have a single strand of DNA and RNA, how...
28 February 2024 1,869 10 View
Is it possible that a hybrid virus is generated in a eukaryotic cell by co-infection of different viruses through nonhomologous recombination?
14 September 2023 7,245 3 View
For diagnostics of Avian infectious bronchitis viruses, I use an end-point RT PCR reaction, and before the RT srage I do a primer annealing stage with the target reverse primer (2uM). My queation...
22 August 2023 1,600 1 View
I am looking for a PhD Position in Microbiology, Virology, and Molecular Biology.
26 July 2023 7,550 1 View
Dear Researchers, I need 2 ml of NZ2 strain ORFV virus for my student Ph thesis. Could you send us, buyer pay. My address: Prof.Dr. Mehmet KALE Department of Virology Faculty of Veterinary...
29 May 2023 7,105 6 View
Hi, I have been reading about vectors and strategies used in engineering insect cell lines. PiggyBac being a very promising system, is not supplied with a autonomously replicating transposase...
17 April 2023 5,873 6 View
I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...
12 January 2023 4,248 2 View
Im trying to find an oligoprimer set for HA gene amplification of H5N1? I will appreciate if you could point out a primer set for phylohenetic analysis by H5N1 HA gene sequencing.
27 November 2022 9,614 2 View