For diagnostics of Avian infectious bronchitis viruses, I use an end-point RT PCR reaction, and before the RT srage I do a primer annealing stage with the target reverse primer (2uM). My queation is: If I have 2 targets, is it right to use a 1:1 mixtue of their reverse primers (2uM) instead of using rendom hexamers, in order to get better sensitivity for these two targets amplified from the same RT product?
Robert Adolf Brinzer
Assuming the sequence heterogeneity is low at your primer binding sites you could replace the random hexamers with your reverse primers.
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