Hi follow virologists,
I'm new to the virology field and I'm eager to understand how freeze/thaw cycles affect virus culture fluid titers. I've noticed that while some viruses show increased titers with multiple freeze/thaw cycles, others don't. I'm curious about why this happens. Also, I've seen that for viruses with an initial increase, the titer eventually drops after a certain number of cycles, why? I am sure it has to do with the different life cycles of different viruses, but I'm looking for a general explanation since I'll be dealing with a wide range of viruses. If it's too much to cover here, I'd appreciate any recommendations on where to find relevant literature.
Thanks a bunch!
CK