What are the universal primer sequnces for H5N1 amplification by RT-PCR (for phylogenetic analysis purpose)?

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Pelleting conditions for Avibacterium paragallinarum (for WGS)?

For WGS, we need to obtain pellets of Avibacterium paragallinarum (Pasteurellacea-like baceria). What are the preffered centrifuge conditions for this bacterial family? Thanks

01 May 2024 8,029 4 View

Why are cells retaining resistance but not desired gene?

We have recently aquired plasmids with the following configuration: EF1A>{gene of interest}:P2A:Bsd When used for transfection (293FT cells and SH-SY5Y cells), these cells expressed the...

11 March 2024 5,049 10 View

Does the centrifugal force due to the Earth's rotation have important meteorological effects?

The components parallel to the ground of the Coriolis force and the centrifugal force, at mid-latitudes (40°N), are respectively 2ωvsenφ≅4.67×10^(-4) ms^(-2), 1/2ω^ 2Rsen( 2φ) ≅1.62×10^(-2)...

20 January 2024 5,475 4 View

RT step- Can I use 2 different target reverse primers instead of random hexamers? For 2 different PCR reactions on the same "first strand" RT product?

For diagnostics of Avian infectious bronchitis viruses, I use an end-point RT PCR reaction, and before the RT srage I do a primer annealing stage with the target reverse primer (2uM). My queation...

22 August 2023 1,600 1 View

How do I measure RNA integrity of pure viral RNA?

In our diagnostic lab, we extract pure viral RNA (Qiagen viral RNA extraction kit). Therefore, no 28S and 18S rRNA can be traced in agarose gel for total RNA integrity assesment. However, very...

24 October 2022 6,952 7 View

Can you reproductively create an end point RT-PCR amplicon product originating at purified viral RNA sample from direct tracheal/cloaca swabs?

Hi to all, We successfully created in our lab a new RT-PCR system, designed for amplification of the S1 segment of Infectious bronchitis virus (IBV). Yet, we obtained the end point PCR product...

27 October 2021 7,947 2 View

Is there a universal qRT-PCR system (Taqman) to detect Infectious bronchitis virus (including QX variants)?

Hello, We have a qRT-PCR system (primers and probe) that does not detect the QX variant. Does anyone knows a universal set of primers and probe for universal detection of IBV? Thanks in...

02 June 2021 7,273 9 View

Which factors or pathways regulates ETV1 expression?

Hello, Which factors or pathways regulates ETV1 expression? Many thanks !! Ido

13 October 2019 7,502 2 View

What will happened if the pH of Ludox TM-50 will be changed from pH9 to pH12 ? Do the silica nanoparticles will be dissolved?

the silica nanoprticles may be dissolved to sodium silicate

07 July 2018 1,467 3 View

What is the best antibody panel for staining PBMCs looking after myeloid-dendritic cells ?

Hi, I need assistance in planning an efficient panel for staining PBMCs in a clinical trial. My plan is to evaluate their activation profile by using FACS analysis after ex vivo stimulation....

09 July 2017 2,664 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,114 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,100 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,428 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,667 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View