How to extract frequencies and mode shapes of a 3D bridge from installed multiple accelero-meter?

Dear all How can I extract the frequencies and mode-shapes of a 3D bridge from the acceleration record of multiple accelero-meters installed on the bridge? Thank you.

04 September 2020 119 6 View

'Modeling Ply ModelingPly.1 is not connected what can cause misaligned solid elements.' !?

Hi everyone, I am trying to model a composite component in ANSYS ACP Pre. After updating ACP I get an error which reads: 'Modeling Ply ModelingPly.1 is not connected what can cause misaligned...

31 May 2020 5,623 1 View

Does anybody have any idea from where to buy (+)-artemisinic acid in gram (10-25-50 g) scale?

Does anybody have any idea from where to buy (+)-artemisinic acid in gram (10-25-50 g) scale? I need this compound (which si actually starting material of artemissinine, antimalarial drug in...

31 October 2019 8,320 4 View

Could anybody recommend me an efficient troubleshooting method in the case of Phusion or Pfu polymerase?

12 August 2016 8,830 2 View

How can I apply a Kruskal-Wallis or a Friedman test for 2 or more between- / within-subject factors?

We repeatedly faced a problem when analyzing data in behavioral studies. In more sophisticated experimental designs, we need to handle more factors (e.g., a pharmacological treatment and a...

24 March 2015 3,595 11 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View