Hello all. I grew ARPE-19 cells in cell culture and infected them with a virus (Varicella-zoster virus). After the virus reached a high infection rate, I harvested everything in the plate and use freeze & thaw technique to release viruses into the supernatant. Now I want to store my viruses in -80 freezer. What is the composition of freezing medium for VZV? Are DMSO and FBS enough? Or do I need to add sucrose or something else? Any advice is appreciated.
Hi,
The freeze media: DMEM (or another suitable cell culture medium), 10-20% FBS, 5-10% DMSO.
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Good luck!
The best option for viruses with a lipid envelope is 50% or more (up to 80%) - bovine serum. The rest: normal MEM medium
Hello Shaokai Pei
Use 0.2M sucrose phosphate as a cryoprotectant for varicella-zoster virus.
You may want to refer to the article attached below.
Article Effect of sucrose phosphate and sorbitol on infectivity of e...
Virus infected cells may be frozen in 70% culture media + 20% FBS + 10% DMSO.
Best.
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