Not sure what you are dealing with since the resolution of the picture is not the greatest (from what I could see I suspect bacteria). Unless these cells are primary cells for which you have no other vial frozen, I would not attempt to clean them. Just start with a new fresh vial of cells.

Not sure what you are dealing with since the resolution of the picture is not the greatest (from what I could see I suspect bacteria). Unless these cells are primary cells for which you have no other vial frozen, I would not attempt to clean them. Just start with a new fresh vial of cells.

As Richard said it's hard to know exactly what the contamination is by the picture, but, if you can, do what Richard said and start with a fresh vial since it's almost impossible to eliminate such a big contamination.

Increasing the antibiotic/antimicotic will kill your cells together with the contamination, but, if it is a primary culture, you can try using differents antibiotic at the same time since if the contamination become resistant to one of them the other would continue killing it.

I think it is too big to be bacteria and it looks like filaments (assuming the big ones are your cells), so I would think the contamination is a fungus. The best thing is to start with a new vial, otherwise you could try some fungicides, but these interfere sometimes with other eukaryotic cells.

What is the name of your cell line?. some cells form a filamentous like outline, but you can see the nucleus. But as everyone said, if you think you have contamination start with new vial and make sure that your media is not contaminated.

my media is ok. it is mx-1 cell (breast cancer cell). my cells is very resistance i think.  a few days with this ,,super,, media i think would not kill cells. the  contamination is really big.... this view took after 24h. before cells looks ok. 

It is a big contamination after 24h, specially if it is fungal. Just keep in mind that the fungicide might affect your cells even if it doesn't kill them, as they usually target ribosomes and such, which always have cross-reaction in other eukaryotes. It all depends on what you want to do with the cells, as the fungicide is going to put stress on them, besides the stress already that they have from the contamination (space restriction, depletion of nutrients etc). Even if they survive, and you get rid of the contamination completely, I would passage them several times before using them in any experiment, as those cells are going to be quite messed up, and you might not get the "real" result or not be able to reproduce it in the future. As everyone has said, if you have other aliquotes of the cells, just take a new one, and just in case, change the media and all the reagents you use for passage, clean the hood very well, and maybe even the incubator (we had a massive fungal contamination in ours). Good luck

Look like bacterial (bacilli) to me. By increasing the concentration of antibiotics probably will not help. I used to have such problem and had tried 10% antibiotics treatment for several hours without any help. Throw it away and find a new batch of cells is my suggestion.

It's very difficult to completely eliminate these kinds of infections and you may never know just how you will be affecting your cells if you increase your antibiotic/fungal concentrations. In other words, even if you manage to clear up the contamination, ask yourself if you truly think you could completely trust your results afterwards. Also, would you get the same results from cells you didn't have to treat? Assuming you want to replicate your results after using the "cleared" cells, do you then treat your next batch of cells with the same increased antibiotic/fungal concentration to keep things constant?

They are bacilli in chain (usually look filamentous and the cells could be smaller than cocci, so hard to separately see individual bacteria). As all mentioned above, If possible discard the culture and if you still want these cells desperately, wash several times, trypsinize and replate with gentamycin and maintain in >=50µg/ml Gentamycin (Be careful about the results you get from them anyway!!!)

Good Luck

hmm..thy are moving bacteria and you can observe them in microscope, moving between your cells. They eat up all your cells.

The best way is to discard the culture and don't use it at all as the bacterial genome can cause unusual and unpredicted menifestations in cell's genome.

Try to maintain sterility and proper huygeine.

Cheers!