Adam M. Passman

5 Questions 14 Answers 0 Followers

Questions related from Adam M. Passman

What R packages can I use to find optimal marker combinations to identify a scRNAseq subcluster?

Let me start by saying I'm very new to the scRNAseq world and am learning my way around R. I now have an scRNAseq dataset and I have identified a subcluster of a cell-type that I'm interested in....

04 April 2024 9,531 0 View

What are differences between air-drying/alcohol/heat-block dehydrating for FFPE IHC?

I've seen various protocols suggesting either drying samples on a heat-block, dehydrating through an increasing EtOH concentration gradient or just air-drying before mounting or storage. What are...

04 June 2018 7,272 3 View

Can anyone recommend an HIF1 reporter that works in mice?

I'm culturing cells in a hypoxia chamber and would like to see if I am inducing HIF1 expression in my mouse cells. Has anyone used an HIF1 reporter in mice that they can recommend?

06 June 2013 377 2 View

Does anyone have experience with the TBARS kit from caymanchem?

I have tried using the TBARS assay kit from caymanchem (10009055) and am not getting the changes I expect to see. For those who have used this kit, do you follow the protocol exactly as provided...

25 February 2013 9,804 1 View

What are some markers of oxidative stress/DNA damage?

I'm in a biochemistry lab and don't have access to mass spectrometry methods. We're set up for immunofluorescence, immunohistochemistry, Western blot etc. Does anyone have a recommendation for a...

21 February 2013 9,581 20 View