Catarina A. Marques

4 Questions 28 Answers 0 Followers

Questions related from Catarina A. Marques

What is the correct way to represent overlayed FACS data when the number of acquired cells is different between samples?

Hello, I have data from an experiment in which I'm comparing cell cycle profiles (PI staining) in treated vs untreated cells. The problem was that in certain cases, the number of treated cells was...

25 July 2015 2,191 6 View

Does anyone know of a mitosis marker in Trypanosoma brucei?

I was wondering if anyone knows of a marker for mitosis in Trypanosoma brucei? I want to be able to identify by IFA 1N2K cells that were already in mitosis, and thus distinguish them from 1N2K...

21 January 2015 9,276 4 View

FACS of Trypanosoma brucei parasites?

I am interested in sort blood stream forms (BSFs) of Trypanosoma brucei. So far I've been successful sorting the procyclic form cells of this parasite using a protocol that has been published...

03 December 2013 6,572 13 View

What is the minimal amount of DNA that can be used for a qPCR reaction?

I need to do some qPCR on qDNA samples that are at very low concentrations, such as 0.5-1 ng/ul. I have very few samples left, and I was wondering if anyone knows the minimum amount of DNA that...

20 November 2013 1,024 17 View