Ajay Kumar

6 Questions 224 Answers 0 Followers

Questions related from Ajay Kumar

How to purify iPSCs after reprogramming?

Dear Friends, I used CytoTune-iPS 2.0 Sendai Reprogramming Kit from Thermo Fisher to make iPSCs from primary human cells. Colonies were generated successfully after three weeks and grew to...

18 July 2018 6,828 9 View

Why kinesin motor domain proteins, intracellular signalling proteins and some receptor proteins are present in secretome?

Hello friends, During proteomic analysis of stem cell secretome, we observed that secretome is significantly enriched for PFAM protein domain (kinesin motor domain). I was also wondering about...

06 June 2017 3,119 3 View

Can anybody in India provide a human embryonic stem cell (hESC) line on urgent basis?

Hello friends, I need a human ESC cell line regarding some urgent work which I need to compile within three months. Is there anyone in India who can deliver me the same. Any substantial help will...

19 October 2016 2,498 4 View

Patch clamp technique in India ?

Hello Friends, I want to measure the voltage/action potential of neurons generated by adult stem cell differentiation. Can anybody suggest any lab in India, preferably North India (in...

13 February 2015 3,287 17 View

What is the best clinically approved MSC media to be used for clinical trials?

Hello everyone, I need details about mesenchymal stem cell (bone marrow stem cell) media which can be used for clinical trial studies and which is clinically approved. Our application can also...

17 December 2014 6,419 18 View

Is anybody familiar with Immunofluorescence staining of neurospheres?

Hello all, I want to do IFS for neurospheres as a whole (to be specific, I dont want to trpsinize neurosphere, make a sss and then use that for IS) What I have observed is that antibody is not...

11 December 2014 4,686 13 View